But, for long-term storage of the powder form, the required temperature is less than -20 degrees celsius. Cookie Notice It could be potentially harmful to try to reconstitute a peptide meant for mixing with bacteriostatic water with sterile water. However, research is not considered conclusive. All rights reserved. Lets nail the major differences right from the start. Many people have this mentality that bacteriostatic water and sterile water are the same thing, and that can bring disastrous results. By rejecting non-essential cookies, Reddit may still use certain cookies to ensure the proper functionality of our platform. In summary, the best way to store reconstituted peptides depends on the duration of storage and the desired shelf life. Now we will explore some of the most exciting applications. The pH is 5.7 (4.5 to 7.0). Dont waste time and energy attempting to source syringes, alcohol swabs, and bacteriostatic water from multiple retailers. Benzyl alcohol, a preservative in Bacteriostatic Water for Injection, USP has been associated with toxicity in neonates. If the peptide still doesnt get dissolved, perform trial and error and use various other solvents. However, some of the other dosage forms that are lyophilized, such as hydrocortisone sodium succinate and methylprednisolone sodium succinate have no antibacterial effect when in solution. But dont get too excited just yet. Once you open the seal of the sterile water vial, you will either have to use it preferably within the next 4 hours or discard it. .063 mg/mL, 3200 mL c. 1000 mg/mL, 5 mL d. 64 mg/mL b. In order to reconstitute 10 equal dosages, we need to mix 1ml of bacteriostatic water in all the 10 injections, which means 0.1ml of water per dosage. The benzyl alcohol in the water makes bacteriostatic water ideal for diluting any drug that will be administered by intramuscular, intravenous, or subcutaneous injection. Yes, distilled water can also be used to reconstitute peptides alongside the bacteriostatic and sterile water. The second step is to use your syringe and draw out 1ml of bacteriostatic water from the vial without really touching the needle as it could cause contamination. These Terms will be governed by and interpreted in accordance with the laws of the State of New York, and you submit to the non-exclusive jurisdiction of the state and federal courts located in New York for the resolution of any disputes. The site is secure. [See USP Controlled Room Temperature. Drug class: Sterile irrigating solutions. Some drugs for injection may be incompatible in a given vehicle, or when combined in the same vehicle or in a vehicle containing benzyl alcohol. Sample 1 Sample 2 Sample 3. Researchers also use organic solvent such as Acetic acid and DMSO to dissolve hydrophobic peptides and polar uncharged amino acids. Wipe down all materials with an alcohol swab. 1996-2023 RxList, Inc. An Internet Brands company. This website is using a security service to protect itself from online attacks. After that, collect and prep your materials. To do the reconstitution you will need: Vial . Medications that required diluting with water usually indicate on the bottle which water is preferred for use. Bacteriostatic water and sterile water can be used in intravenous, intramuscular and subcutaneous injections. Only if the peptides are of the same nature such as growth hormone (GH) influencers or if you see any vendors selling a stack of particular peptides, only those you should mix. Please include what you were doing when this page came up and the Cloudflare Ray ID found at the bottom of this page. There are only two kinds of water that are most safe to reconstitute peptides. Due to the lack of antimicrobials, sterile water can easily be contaminated once exposed to surroundings and even yourself. Your best option for assembling the necessary tool kit is a single, reputable source. There is literally no other option. Parenteral preparations containing benzyl alcohol should not be used in epidural or spinal anesthesia procedures. Bacteriostatic Water for Injection, USP must be made approximately isotonic prior to use. Another method of sterilization that has been practiced is the use of gaseous ethylene oxide. Before really using the peptide, you will have to add liquid to the powder and MIX it well to make it a solution but it isnt as simple as it sounds! Gather the following: alcohol swabs, bacteriostatic water, peptide powder, and a sterile syringe. All you have to do is add the desirable reconstituted peptides in 1 vial and then use the syringe to pull out the mixture so that you can administer it. They both are nonpyrogenic meaning they wont cause fever. The starter set has all you need to get going, including the following: If you want a little more, check out the premium kit: Qualified handlers are sure to enjoy this top online source for research materials. Lets explore their points of similarity and difference to understand their appropriate uses. Plus, youll feel like a mad scientist, and who doesnt love that feeling? Bacteriostatic Water for Injection Prescribing Information. Statements regarding products presented on Peptides.org are the opinions of the individuals making them and are not necessarily the same as those of Peptides.org. Recommended dosage guidelines are usually based on either the clinical trials or the efficacy of the peptide. 1. Pregnancy: Animal reproduction studies have not been conducted with Bacteriostatic Water for Injection. Privacy Policy. It also is prepared with a bacteriostat, a compound used to prevent any bacterial growth. Bacteriostatic Water for Injection, USP is a sterile, nonpyrogenic preparation of water for injection containing 0.9% (9 mg/mL) of benzyl alcohol added as a bacteriostatic preservative. Reconstitution means any Securitization Transaction or Whole Loan Transfer. Bacteriostatic Water is a prescription medicine used to dilute or dissolve medications. Dosage form: injection, solution Water for Injection, USP is chemically designated H2O. Because most peptides are sold in powder form, one needs to dissolve/dilute them before really using them. Made with Muscle. But not all peptides dissolve in water easily, so it usually is recommended that you try to dissolve the peptide in any of these waters using Sonication. Data are unavailable on the toxicity of other preservatives in this age group. It is considered an ideal sterile solution due to its low toxicity and minimal interference with drug effectiveness. Once you have put the details, our reconstitution calculator will use its algorithm and devise the volume needed to get your peptides reconstituted! As with any ethylene oxide treatment, humidification is necessary. [See USP Controlled Room Temperature. Our goal is to provide you with the most scientifically accurate, unbiased, and comprehensive information regarding all research peptides and SARMs. According to a recent study, 99.9% of people struggle with this step. The benzyl alcohol in it would cause red blood cell lysis. Our focus is to exclusively link to peer-reviewed studies found on respected websites, like PubMed. Due to the potential for toxicity, solutions containing benzyl alcohol should not be used in neonates. These formulations are less expensive to produce and more widely available than comparable products, such as sterile water [3]. The container can be re-entered multiple times (usually by a sterile needle) and the benzyl alcohol is added to ensure that bacteria does not grow in the water as diluting medications with tainted water can have some severe consequences. Long-term storage: For long-term storage, it is recommended to aliquot the reconstituted peptide into small volumes and freeze them at -20C or -80C. Subscribe to Drugs.com newsletters for the latest medication news, new drug approvals, alerts and updates. Bacteriostatic water is very easy to manufacture while sterile water is an arduous process. Morgan, SL and Spotts, MR; "How to Select a Pharmaceutical Freeze-Drying System"; Pharmaceutical Technology, volume 3, No. When reconstituted with Bacteriostatic Water for Injection, USP, the resulting solution must be used within 7 days. When preparing bacteriostatic water for injection, all product indications should be closely followed, including recommended dosages and injection schedules [4]. Additionally, stability data should include provisions for the assay of aged samples and subsequent reconstitution of these aged samples for the maximum amount of time specified in the labeling. Both sterile solutions are formulated for medical use. What is the resulting supply dose in mg/mL? An official website of the United States government, : On the other hand, TB-500, another potential peptide for quick healing is usually dosed at 7.66mg per week. Although products may be labeled for reconstitution with Bacteriostatic Water for Injection, Sterile Water for Injection (WFI) should be used to reconstitute products. and our Then you're in luck! I need to rehydrate some BPC, but when I look for bacteriostatic water on Amazon, it takes me directly to "reconstitution solution." Gently remove the needle from the vial. It is essential to avoid exposure to light and high temperatures, which can lead to peptide degradation. If the product is reconstituted with Sterile Water for Injection, USP, use immediately and discard any unused portion. Inspect reconstituted (diluted or dissolved) drugs for clarity (if soluble) and freedom from unexpected precipitation or discoloration prior to administration. Click to reveal When the lyophilized product does not include an excipient or other additive and only represents the active ingredient, weight variation can be employed as the means to test for dose uniformity. Parenteral preparations with benzyl alcohol should not be used for fluid replacement. The sterilization of the lyophilizer is one of the most frequently encountered problems. Although rarely, but they can occur, those side effects are more related to the drug that is dissolved in the bacteriostatic water. To really know the best solvent in which a peptide will be soluble, you must have to consider some biochemistry knowledge and do some trial and error. EZCORE INTERNATIONAL PVT. 1) Determine the quantity of peptide, in micrograms (mcg), in your vial. These Terms will be applied fully and affect to your use of this Website. Water distribution depends primarily on the concentration of electrolytes in the body compartments and sodium (Na+) plays a major role in maintaining physiologic equilibrium. Certain areas of this Website are restricted from being access by you and Buckle LLC may further restrict access by you to any areas of this Website, at any time, in absolute discretion. Besides neonates, they both can be used for the rest of the population including pediatrics, adults and the geriatric community. Sterilization procedures should parallel that of an autoclave and a typical system should include: Provisions for sterilizing the shelf support rods unless they are only exposed to the inside of the chamber after stoppering. Plus, peptide solutions made with bacteriostatic water resist contamination. Use aseptic technique for single or multiple entry and withdrawal from all containers. This process is often helpful in breaking down the big solid lumps of peptides in the water and aids in the dissolution process. Unfortunately, piping such as that for the administration of inert gas (usually nitrogen) and sterile air for backfill or vacuum break are often inaccessible to such surface "sterilization" or treatment. So it all differs and the exact dosage can only be prescribed by your health practitioner. How does the math work when reconstituting a peptide with 5mg of two different peptides in the same vial? The primary uses of bacteriostatic water are as follows: When using bacteriostatic water for injections, certain precautions must be followed. If an adverse reaction does occur, discontinue the infusion, evaluate . The Buckle LLC is allowed to assign, transfer, and subcontract its rights and/or obligations under these Terms without any notification. Complement your research with this USP solvent, the go-to solution of the medical and scientific community. The filling of vials that are to be lyophilized has some problems that are somewhat unique. a. The recommended dosage of BPC 157 is somewhere between 200mcg to 1000mcg, depending on your body weight. 3, pp. This material is provided for educational purposes only and is not intended for medical advice, diagnosis or treatment. If you are a peptide researcher or laboratory professional, you can safely enjoy the benefits of bacteriostatic water with proper use. The next step is to do is add the mass of the peptide in mg that you want to reconstitute (This is generally going to be listed on the peptide vial) with the x ml of bacteriostatic water in order to get your desirable dosage in mcg. Most peptides that you will see are often sold as lyophilized peptides which means that (freeze-dried) powder form. On the other hand, a sterile water vial doesnt contain any preservatives/additives which can keep it free of pathogens for a longer time, thus one will have to use it immediately after opening the seal. swelling of your face, lips, tongue, or throat, redness, warmth, and pain at the injection site, tenderness or irritation at the injection site. and discoloration prior to administration, whenever solution and container permit. Bacteriostatic 0.9% Sodium Chloride (NaCl) Injection, USP is a sterile, nonpyrogenic, isotonic solution of sodium chloride in water for injection. The process of reconstitution is the same regardless of what kind of water you chose to reconstitute your AOD with. You must not use this Website if you disagree with any of these Website Standard Terms and Conditions. You are granted limited license only for purposes of viewing the material contained on this Website. While there is not a long-term shortage, an acute shortage is often seen because of either manufacturing facilities malfunction or some medical emergency such as COVID-19. Beisswenger, H.L. The safety of the plastic has been confirmed by tests in animals according to USP biological standards for plastic containers. This parenteral preparation is unlikely to 100 mg Plain. On the other hand, distilled water is rather more sterilized and contains neither organic nor organic materials. 29-31, 48; 1986. Often available as a lyophilized powder, hCG is best reconstituted with bacteriostatic water for injection due to the solvents efficiency and preservative agents. It is only used for injections such as intravenous, intramuscular and subcutaneous. Differences Between Bacteriostatic Water vs. Sterile Water Reviewed in the United States on January 28, 2023. Both have similar uses and are sterile but have drastically different demographics and indications. In order to provide guidance and information to investigators, some industry procedures and deficiencies associated with lyophilized products are identified in this ITG. If both items are the same (benzyl alcohol bacteriostatic solutions) why use the different name? Drop us a comment down below and one of our expert coaches will respond to it, you can also email is at coaching@muscleandbrawn.com. 3) Choose the size of the syringe you want . Do not store reconstituted solutions of drugs for injection unless otherwise directed by the manufacturer of the solute. Administration of an estimated 9 mL to a 6 kg infant or neonate is potentially capable of producing blood pressure changes. Peptide Dosing Guide. What is bacteriostatic water? A separate formulation is used for irrigation such as washing, rinsing and diluting. Store at 20 to 25C (68 to 77F). The next and immediate step is to add the water you have drawn out into the peptide vial slowly yet smoothly. Lets take a look at each one, where they differ, and where they overlap. Water distribution depends primarily on the concentration of electrolytes in the body compartments and sodium (Na+) plays a major role in maintaining physiologic equilibrium. Bacteriostatic Water for Injections | The A-Z Guide, Add a header to begin generating the table of contents. If available, sonication may be performed in order to speed up the process.
